Flex v2 cost calculator

Estimate reagent and sequencing costs for GEM-X Flex v2 experiments.

Experiment inputs

Recovered cells needed100,000 25,00010,000,000 Acceptable undetected doublet rate1.0% 0.2%20%

Rates above 8% extrapolate the approximate 0.4% per 1,000-cell relationship beyond the published 20,000-cell-per-barcode range.

Hybridization reactions

GEM lanes

Prices and technical assumptions

Base kit

Extra 4-lane pack

Solaris wafer

Reads per cell

Reads per wafer

Recovery estimate

20Hybridization reactions

2GEM lanes

1Solaris wafers

Configuration capacity100,000 cells

Target covered

Allocated experiment cost$8,700$0.09 per recovered cell

Allocated Flex reagents$6,000

Sequencing$2,700

Upfront purchase cost$26,700

Partial plate use is allocated in 12-well blocks at one-eighth of the base-kit price.
Additional gel bead reactions are purchased in four-reaction packs.
Sequencing is rounded up to whole wafers.
Estimates exclude probes, chips, indices, labor, taxes, discounts, and failed runs.