ProbeMaker

Generate complementary sequences to mRNA for your genes

Tip: You can paste a list of gene names from Excel or any text file. Maximum 100 genes per request.
Important: Please verify specificity and directionality of your probes through BLAST or equivalent tools before ordering.

Scientific Accuracy

Generates reverse complementary sequences with proper DNA/RNA transcription

Probe Design

Creates 50-base probes split into LHS/RHS 25-base segments

NCBI Integration

Automatically fetches gene sequences from NCBI databases

Quality Constraints

Enforces GC content, position constraints, and homopolymer limits

API Usage

This tool can also be used programmatically via API endpoints:

POST /generate
Content-Type: application/x-www-form-urlencoded

gene_names=BRCA1%0ATP53%0AEGFR

Note: This standalone version requires a backend server to function. For full functionality, deploy the Flask app or use the command-line version.