Phospho-seq
Phospho-seq
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3
Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators [FlexPlex]
John D. Blair
,
Alexandra Bradu
,
Carol Dalgarno
,
Isabella N. Grabski
,
Rahul Satija
PDF
BioRxiv
Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression [Phospho-seq]
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno, Barbara Treutlein, Rahul Satija
PDF
Final Publication
BioRxiv
Virtual Seminar
Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting
Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting [FlexPlex]
Isabella N. Grabski
,
Junsuk Lee
,
John Blair
,
Carol Dalgarno
,
Isabella Mascio
,
Alexandra Bradu
,
David A. Knowles
,
Rahul Satija
PDF
BioRxiv
Single-cell genotype-phenotype mapping identifies therapeutic vulnerabilities in VEXAS syndrome
Single-cell genotype-phenotype mapping identifies therapeutic vulnerabilities in VEXAS syndrome [Phospho-seq]
Saravanan Ganesan
,
Rebecca M. Murray
,
Jesus Sotelo
,
Elliot O. Eton
,
Kouhei Takashima
,
Theo Botella
,
Kai Beattie
,
Alyssa C. Indart
,
Nada Chraiki
,
Carolyne Croizier
,
Franco Izzo
,
Catherine Potenski
,
Samuele Marro
,
Zhijie Wu
,
Shouguo Gao
,
Neal S. Young
,
John D. Blair
,
Rahul Satija
,
Benjamin Terrier
,
Mael Heiblig
,
Eirini P Papapetrou
,
Pierre Sujobert
,
Olivier Kosmider
,
David B. Beck
,
Dan A. Landau
PDF
BioRxiv
Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno,Barbara Treutlein, Rahul Satija
Last updated on Aug 16, 2023
PDF
DOI
BioRxiv
Virtual Seminar
AIPL1
Antibody description
Last updated on Nov 27, 2023
Link to Vendor
AMPK
Antibody description
Last updated on Nov 19, 2023
Link to Vendor
Analyzing Phospho-seq Data with Signac and Seurat
A basic guide to processing Phospho-seq data with Signac and Seurat, including useful visualizations.
Last updated on Oct 24, 2025
R Script
Related Reference - Signac
Signac
Seurat
Antibody Conjugation
A guide to conjugating antibodies with TCO-labeled oligos, as provided in the Phospho-seq kit.
Last updated on Oct 24, 2025
PDF
Original Reference
Protocol with Oligo Labeling
Bactin
Antibody description
Last updated on Nov 19, 2023
Link to Vendor - Brain Organoid
Link to Vendor - Retinal Organoid
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