Phospho-seq
Phospho-seq
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Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators [FlexPlex]
John D. Blair
,
Alexandra Bradu
,
Carol Dalgarno
,
Isabella N. Grabski
,
Rahul Satija
PDF
BioRxiv
Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression [Phospho-seq]
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno, Barbara Treutlein, Rahul Satija
PDF
Final Publication
BioRxiv
Virtual Seminar
Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting
Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting [FlexPlex]
Isabella N. Grabski
,
Junsuk Lee
,
John Blair
,
Carol Dalgarno
,
Isabella Mascio
,
Alexandra Bradu
,
David A. Knowles
,
Rahul Satija
PDF
BioRxiv
Single-cell genotype-phenotype mapping identifies therapeutic vulnerabilities in VEXAS syndrome
Single-cell genotype-phenotype mapping identifies therapeutic vulnerabilities in VEXAS syndrome [Phospho-seq]
Saravanan Ganesan
,
Rebecca M. Murray
,
Jesus Sotelo
,
Elliot O. Eton
,
Kouhei Takashima
,
Theo Botella
,
Kai Beattie
,
Alyssa C. Indart
,
Nada Chraiki
,
Carolyne Croizier
,
Franco Izzo
,
Catherine Potenski
,
Samuele Marro
,
Zhijie Wu
,
Shouguo Gao
,
Neal S. Young
,
John D. Blair
,
Rahul Satija
,
Benjamin Terrier
,
Mael Heiblig
,
Eirini P Papapetrou
,
Pierre Sujobert
,
Olivier Kosmider
,
David B. Beck
,
Dan A. Landau
PDF
BioRxiv
Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno,Barbara Treutlein, Rahul Satija
Last updated on Aug 16, 2023
PDF
DOI
BioRxiv
Virtual Seminar
Analyzing Phospho-seq Data with Signac and Seurat
A basic guide to processing Phospho-seq data with Signac and Seurat, including useful visualizations.
Last updated on Oct 24, 2025
R Script
Related Reference - Signac
Signac
Seurat
Antibody Conjugation
A guide to conjugating antibodies with TCO-labeled oligos, as provided in the Phospho-seq kit.
Last updated on Oct 24, 2025
PDF
Original Reference
Protocol with Oligo Labeling
FlexPlex
A guide to running FlexPlex, which quantifies protein, guides, oligohashes and RNA using the 10x Flex kit.
Last updated on Oct 24, 2025
PDF
Related Reference
ADT, OligoHash and Guide Quantification Script
Phospho-seq with 10X ATAC-seq kit
A guide to running Phospho-seq with the 10x scATAC kit.
admin
Last updated on Oct 24, 2025
PDF
Related Reference
Phospho-seq with the 10x Multiome Kit
A guide for running Phospho-seq using the 10x Multiome kit to capture RNA in addition to ATAC and Protein.
Last updated on Oct 24, 2025
PDF
Related Reference
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