Phospho-seq
Phospho-seq
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Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno,Barbara Treutlein, Rahul Satija
Last updated on Aug 16, 2023
PDF
DOI
BioRxiv
Virtual Seminar
Phospho-seq: Integrated, multi-modal profiling of intracellular protein dynamics in single cells
Phospho-seq is a multimodal single cell genomics method to measure intracellular proteins, chromatin accessibility and gene expression
John D. Blair, Austin Hartman, Fides Zenk, Carol Dalgarno, Barbara Treutlein, Rahul Satija
PDF
BioRxiv
Virtual Seminar
Analyzing Phospho-seq Data with Signac and Seurat
A basic guide to processing Phospho-seq data with Signac and Seurat, including useful visualizations.
Last updated on Nov 6, 2023
R Script
Related Reference - Signac
Signac
Seurat
Antibody Conjugation
A guide to conjugating antibodies with TCO-labeled oligos, as provided in the Phospho-seq kit.
Last updated on Nov 6, 2023
PDF
Original Reference
Protocol with Oligo Labeling
Phospho-seq with 10X ATAC-seq kit
A guide to running Phospho-seq with the 10x scATAC kit.
admin
Last updated on Nov 3, 2023
PDF
Related Reference
Phospho-seq with the 10x Multiome Kit
A guide for running Phospho-seq using the 10x Multiome kit to capture RNA in addition to ATAC and Protein.
Last updated on Nov 6, 2023
PDF
Related Reference
Preparing Antibodies for Conjugation
A quick protocol to clean up antibodies with Promega Magne Beads to prepare them for conjugation with the Phospho-seq kit.
Last updated on Nov 6, 2023
PDF
Antibody Purification Beads
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