Protocols

A basic guide to processing Phospho-seq data with Signac and Seurat, including useful visualizations.

A guide to conjugating antibodies with TCO-labeled oligos, as provided in the Phospho-seq kit.

A guide to running FlexPlex, which quantifies protein, guides, oligohashes and RNA using the 10x Flex kit.

A guide to running Phospho-seq with the 10x scATAC kit.

A guide for running Phospho-seq using the 10x Multiome kit to capture RNA in addition to ATAC and Protein.

A quick protocol to clean up antibodies with Promega Magne Beads to prepare them for conjugation with the Phospho-seq kit.

Helpful tools for designing Phospho-seq and FlexPlex experiments